Journal: Developmental Cell

Article Title: Chk1 Inhibition of the Replication Factor Drf1 Guarantees Cell-Cycle Elongation at the Xenopus laevis Mid-blastula Transition

doi: 10.1016/j.devcel.2017.06.010

Figure Lengend Snippet:

Article Snippet: Mouse monoclonal anti-Myc antibody (9E10) coated magnetic beads , Origene , (cat # TA150044).

Techniques: Concentration Assay, Magnetic Beads, Recombinant, FLAG-tag, Software

Journal: PLoS ONE

Article Title: The HCV Non-Nucleoside Inhibitor Tegobuvir Utilizes a Novel Mechanism of Action to Inhibit NS5B Polymerase Function

doi: 10.1371/journal.pone.0039163

Figure Lengend Snippet: A) NS5B, NS5A, and NS3 Western blots of lysates from 1b (Con-1) replicon cells treated with 50 nM TGV for various periods of time. B) NS5B Western blot of lysates from 1b replicon cells treated for 24 hours with known HCV inhibitors at 50× EC 50 concentrations. C) NS5B Westerns of cell lysates collected at various times from Huh-luc 1a replicon cells treated with 50 nM or 500 nM TGV and lysates from HeLa 1b replicon cells treated with 10 µM TGV. D) TGV EC 50 values in Huh-luc 1a, 1b, and HeLa 1b (clone SL3) replicon cell lines. E) Western blot of lysates from Lunet cells transiently transfected with wild-type or NS5B Y448H mutant 1b Pi-Rluc replicons and treated with varying concentrations of TGV for 24 hours.

Article Snippet: Mouse anti-NS5B (clone 3B1) antibody (Axxora), mouse anti-NS5A antibody (Apath), and mouse anti-NS3 antibody (Virostat) were used as primary antibodies for detection of NS5B, NS5A, and NS3, respectively.

Techniques: Western Blot, Transfection, Mutagenesis